Ride comfortably and confidently knowing you're traveling with Minnesota's best transportation company of 2025! Jefferson Lines is Your #1 Bus Experience in Minnesota for over 105 years. Jefferson Lines provides convenient, reliable, and affordable transportation throughout 14 states with connections to over 2,000 destinations within the U.S. including direct routes to the Twin Cities and Iron Range. Passengers experience safe and clean travel while aboard our luxury motorcoaches. Our full-service Duluth Depot conveniently located downtown with additional bus stops at the University of Minnesota Duluth and The College of St. Scholastica.